IFN Induced GTPase-like Protein Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: IFN Induced GTPase-like Protein Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-100-401-N95
• Hersteller Artikelnummer: 100-401-N95
• Alternativnummer: ROC-100-401-N95
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Mouse
• Immunogen: IFN Induced GTPase Like Protein was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal region of mouse IFN Induced GTPase Like Protein.
• Konjugation: Unconjugated
• Alternative Synonym: rabbit anti-IFN Induced GTPase-like Protein Antibody, interferon-gamma induced, Interferon-gamma-inducible GTPase Ifgga3 protein

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 80 mg/mL by Refractometry
• NCBI: 225594
• UniProt: E9QAA8
• Puffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Mouse
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:2000-1:10000, WB: 1:500-1:1000
• Anwendungsbeschreibung: Anti-IFN Induced GTPase Like Protein has been tested by ELISA and Western Blot. Expect a band at ~48 kDa by western blotting in the appropriate cell lysate or extract.

Bilder

Western Blot of Rabbit Anti-IFN GTPase antibody. Lane 1: WT mouse spleen lysate. Lane 2: IFN gamma gene KO mouse spleen lysate. Load: 35 µg per lane. Primary antibody: IFN GTPases antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: [KO gene results in chronic and persistent IFN gamma protein in the sera] ~52kDa for IFN GTPases antibody. Other band(s): nonspecific.