IFN Induced GTPase-like Protein Antibody, Rabbit, Polyclonal
Biozol Catalog Number:
ROC-100-401-N95
Supplier Catalog Number:
100-401-N95
Alternative Catalog Number:
ROC-100-401-N95
Manufacturer:
Rockland Immunochemicals
Host:
Rabbit
Category:
Antikörper
Application:
ELISA, WB
Species Reactivity:
Mouse
Immunogen:
IFN Induced GTPase Like Protein was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal region of mouse IFN Induced GTPase Like Protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-IFN Induced GTPase-like Protein Antibody, interferon-gamma induced, Interferon-gamma-inducible GTPase Ifgga3 protein
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Mouse
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:2000-1:10000, WB: 1:500-1:1000
Application Notes:
Anti-IFN Induced GTPase Like Protein has been tested by ELISA and Western Blot. Expect a band at ~48 kDa by western blotting in the appropriate cell lysate or extract.
Western Blot of Rabbit Anti-IFN GTPase antibody. Lane 1: WT mouse spleen lysate. Lane 2: IFN gamma gene KO mouse spleen lysate. Load: 35 µg per lane. Primary antibody: IFN GTPases antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: [KO gene results in chronic and persistent IFN gamma protein in the sera] ~52kDa for IFN GTPases antibody. Other band(s): nonspecific.
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