NFkB p50 Antibody, Rabbit, Polyclonal

Artikelnummer: ROC-100-4164S
Artikelname: NFkB p50 Antibody, Rabbit, Polyclonal
Artikelnummer: ROC-100-4164S
Hersteller Artikelnummer: 100-4164S
Alternativnummer: ROC-100-4164S
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Human NFkB p50 (NFKB1) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Konjugation: Unconjugated
Alternative Synonym: rabbit Anti-NFKB p50 antibody, rabbit anti-NFKB1 antibody, NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB
Klonalität: Polyclonal
Konzentration: 80 mg/ml by Refractometry
NCBI: 4790
UniProt: P19838
Puffer: None
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Human
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:2,000
Anwendungsbeschreibung: This product was tested by immunoblot and ELISA. Anti-Human NFkB p50 (NFKB1) is suitable for the detection by immunoblot of Human NFkB p50 (NFKB1) and its precursor protein p105, and was found to be reactive against Human NFkB p50 (NFKB1) at a dilution o
Western Blot of Anti-NFKB p50 (NFKB1) Antibody. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5 each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.