NFkB p50 Antibody, Rabbit, Polyclonal

Catalog Number: ROC-100-4164S
Article Name: NFkB p50 Antibody, Rabbit, Polyclonal
Biozol Catalog Number: ROC-100-4164S
Supplier Catalog Number: 100-4164S
Alternative Catalog Number: ROC-100-4164S
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Human NFkB p50 (NFKB1) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugation: Unconjugated
Alternative Names: rabbit Anti-NFKB p50 antibody, rabbit anti-NFKB1 antibody, NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB
Clonality: Polyclonal
Concentration: 80 mg/ml by Refractometry
NCBI: 4790
UniProt: P19838
Buffer: None
Form: Liquid (sterile filtered)
Target: Human
Antibody Type: Primary Antibody
Application Dilute: ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:2,000
Application Notes: This product was tested by immunoblot and ELISA. Anti-Human NFkB p50 (NFKB1) is suitable for the detection by immunoblot of Human NFkB p50 (NFKB1) and its precursor protein p105, and was found to be reactive against Human NFkB p50 (NFKB1) at a dilution o
Western Blot of Anti-NFKB p50 (NFKB1) Antibody. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5 each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.