NFkB p65 Antibody, Rabbit, Polyclonal

Artikelnummer: ROC-100-4165N
Artikelname: NFkB p65 Antibody, Rabbit, Polyclonal
Artikelnummer: ROC-100-4165N
Hersteller Artikelnummer: 100-4165N
Alternativnummer: ROC-100-4165N
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: WB
Spezies Reaktivität: Human
Immunogen: Anti-NFkB p65 Antibody was produced by repeated immunizations with a synthetic NFkB p65 peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Konjugation: Unconjugated
Alternative Synonym: rabbit Anti-NFKB p65 antibody, rabbit Anti-Rel A antibody, NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB
Klonalität: Polyclonal
Konzentration: 82 mg/mL by Refractometry
NCBI: 5970
UniProt: Q04206
Puffer: None
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Human
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:5,000 - 1:25,000, WB: 1:5000 - 1:10,000
Anwendungsbeschreibung: Anti-NFkB p65 (N-terminal specific) has been tested for the detection of human NFkB p65 (N-terminal specific) by immunoblot. Functionality in supershift assays has not been determined.
Western Blot of Rabbit anti-NFKB p65 N term antibody. Lane 1-4: PC3 cell lysate (p/n W09-001-GV6). Load: 20, 30, 40, and 50 ug per lane. Primary antibody: NFKB p65 antibody at 1:5000 for overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~65kDa for NFKB p65. Other band(s): ~110kDa and ~45kDa.