NFkB p65 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: NFkB p65 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-100-4165N
• Supplier Catalog Number: 100-4165N
• Alternative Catalog Number: ROC-100-4165N
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Human
• Immunogen: Anti-NFkB p65 Antibody was produced by repeated immunizations with a synthetic NFkB p65 peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Conjugation: Unconjugated
• Alternative Names: rabbit Anti-NFKB p65 antibody, rabbit Anti-Rel A antibody, NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB

Product Properties

• Clonality: Polyclonal
• Concentration: 82 mg/mL by Refractometry
• NCBI: 5970
• UniProt: Q04206
• Buffer: None
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:25,000, WB: 1:5000 - 1:10,000
• Application Notes: Anti-NFkB p65 (N-terminal specific) has been tested for the detection of human NFkB p65 (N-terminal specific) by immunoblot. Functionality in supershift assays has not been determined.

Images

Western Blot of Rabbit anti-NFKB p65 N term antibody. Lane 1-4: PC3 cell lysate (p/n W09-001-GV6). Load: 20, 30, 40, and 50 ug per lane. Primary antibody: NFKB p65 antibody at 1:5000 for overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~65kDa for NFKB p65. Other band(s): ~110kDa and ~45kDa.