Thyroglobulin Antibody, IgG1, Clone: [2H11], Mouse, Monoclonal

Artikelnummer: ROC-200-301-388
Artikelname: Thyroglobulin Antibody, IgG1, Clone: [2H11], Mouse, Monoclonal
Artikelnummer: ROC-200-301-388
Hersteller Artikelnummer: 200-301-388
Alternativnummer: ROC-200-301-388
Hersteller: Rockland Immunochemicals
Wirt: Mouse
Kategorie: Antikörper
Applikation: WB
Spezies Reaktivität: Canine, Human, Mouse, Rat
Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with human thyroglobulin protein.
Konjugation: Unconjugated
Alternative Synonym: mouse anti-Thyroglobulin Antibody, AITD 3 antibody, AITD3 antibody, hTG antibody, TDH3 antibody, Tg antibody, TGN antibody
Klonalität: Monoclonal
Konzentration: 1.6 mg/ml by UV absorbance at 280 nm
Klon-Bezeichnung: [2H11]
Isotyp: IgG1
NCBI: 7038
UniProt: P01266
Puffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Human
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:20,000 - 1:100,000, IHC: 1:50 - 1:200, WB: 1:50 - 1:200
Anwendungsbeschreibung: Anti-Thyroglobulin antibody has been tested by western blot and is suitable for the detection of thyroglobulin in ELISA, immunohistochemistry, and immunoprecipitation. For immunohistochemistry, both frozen sections and formalin fixed, paraffin-embedded t
Western blot using ROCKLAND Immunochemicals Mouse Mab-anti-Thyroglobulin antibody. Separation was achieved under reducing conditions using a pre-cast 5% Tris-HCl gel from Bio-Rad Laboratories. This antibody recognizes a single 330 kDa band corresponding to human thyroglobulin (left lane 3 µg, right lane 3 ng) as confirmed by the position of molecular weight markers (not shown). A 1:400 dilution of Mab anti-Thyroglobulin was used for 2h followed by detection using a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Mouse IgG [H&L] (610-132-121) and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.