Anti-Thyroglobulin antibody has been tested by western blot and is suitable for the detection of thyroglobulin in ELISA, immunohistochemistry, and immunoprecipitation. For immunohistochemistry, both frozen sections and formalin fixed, paraffin-embedded t
Western blot using ROCKLAND Immunochemicals Mouse Mab-anti-Thyroglobulin antibody. Separation was achieved under reducing conditions using a pre-cast 5% Tris-HCl gel from Bio-Rad Laboratories. This antibody recognizes a single 330 kDa band corresponding to human thyroglobulin (left lane 3 µg, right lane 3 ng) as confirmed by the position of molecular weight markers (not shown). A 1:400 dilution of Mab anti-Thyroglobulin was used for 2h followed by detection using a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Mouse IgG [H&L] (610-132-121) and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.
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