Thyroglobulin Antibody, IgG1, Clone: [2H11], Mouse, Monoclonal

Product Overview

• Article Name: Thyroglobulin Antibody, IgG1, Clone: [2H11], Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-301-388
• Supplier Catalog Number: 200-301-388
• Alternative Catalog Number: ROC-200-301-388
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: WB
• Species Reactivity: Canine, Human, Mouse, Rat
• Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with human thyroglobulin protein.
• Conjugation: Unconjugated
• Alternative Names: mouse anti-Thyroglobulin Antibody, AITD 3 antibody, AITD3 antibody, hTG antibody, TDH3 antibody, Tg antibody, TGN antibody

Product Properties

• Clonality: Monoclonal
• Concentration: 1.6 mg/ml by UV absorbance at 280 nm
• Clone Designation: [2H11]
• Isotype: IgG1
• NCBI: 7038
• UniProt: P01266
• Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:50 - 1:200, WB: 1:50 - 1:200
• Application Notes: Anti-Thyroglobulin antibody has been tested by western blot and is suitable for the detection of thyroglobulin in ELISA, immunohistochemistry, and immunoprecipitation. For immunohistochemistry, both frozen sections and formalin fixed, paraffin-embedded t

Images

Western blot using ROCKLAND Immunochemicals Mouse Mab-anti-Thyroglobulin antibody. Separation was achieved under reducing conditions using a pre-cast 5% Tris-HCl gel from Bio-Rad Laboratories. This antibody recognizes a single 330 kDa band corresponding to human thyroglobulin (left lane 3 µg, right lane 3 ng) as confirmed by the position of molecular weight markers (not shown). A 1:400 dilution of Mab anti-Thyroglobulin was used for 2h followed by detection using a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Mouse IgG [H&L] (610-132-121) and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.