0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:500 - 1:10,000, WB: 0.5-1 µg/mL
Anwendungsbeschreibung:
Anti-Lin28 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 23 kDa in Western Blots of specific cell lysates and tissues. Validated in h
Western Blot of Mouse anti-Lin28 antibody. Lane A: Raji cell lysate in absence of blocking peptide. Lane B: Raji cell lysate in presence of blocking peptide. Primary antibody: Lin28 antibody at 0.5µg/mL overnight at 4°C. Secondary antibody: Mouse HRP secondary antibody. Block: 5% BLOTTO. Predicted/Observed size: 22 kDa, 23 kDa for Lin28.
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