LIN28 Antibody [1G9H9], IgG2b, Mouse, Monoclonal

Catalog Number: ROC-200-301-CL0
Article Name: LIN28 Antibody [1G9H9], IgG2b, Mouse, Monoclonal
Biozol Catalog Number: ROC-200-301-CL0
Supplier Catalog Number: 200-301-CL0
Alternative Catalog Number: ROC-200-301-CL0
Manufacturer: Rockland Immunochemicals
Host: Mouse
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Anti-Lin28 antibody was produced in mice by repeated immunizations with a 15 amino acid peptide near the C-terminus of human Lin28.
Conjugation: Unconjugated
Alternative Names: LIN28 Antibody, CSDD1, LIN-28, ZCCHC1, lin-28A
Clonality: Monoclonal
Concentration: 1 mg/mL by UV absorbance at 280 nm
Clone Designation: [1G9H9]
Isotype: IgG2b
NCBI: 79727
UniProt: Q9H9Z2
Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form: Liquid (sterile filtered)
Target: Human
Antibody Type: Primary Antibody
Application Dilute: ELISA: 1:500 - 1:10,000, WB: 0.5-1 µg/mL
Application Notes: Anti-Lin28 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 23 kDa in Western Blots of specific cell lysates and tissues. Validated in h
Western Blot of Mouse anti-Lin28 antibody. Lane A: Raji cell lysate in absence of blocking peptide. Lane B: Raji cell lysate in presence of blocking peptide. Primary antibody: Lin28 antibody at 0.5µg/mL overnight at 4°C. Secondary antibody: Mouse HRP secondary antibody. Block: 5% BLOTTO. Predicted/Observed size: 22 kDa, 23 kDa for Lin28.