FTO Antibody, Clone: [5-2H10], Mouse, Monoclonal

Artikelnummer: ROC-200-301-D41
Artikelname: FTO Antibody, Clone: [5-2H10], Mouse, Monoclonal
Artikelnummer: ROC-200-301-D41
Hersteller Artikelnummer: 200-301-D41
Alternativnummer: ROC-200-301-D41
Hersteller: Rockland Immunochemicals
Wirt: Mouse
Kategorie: Antikörper
Applikation: WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Anti-FTO Monoclonal Antibody was produced in mouse by repeated immunizations with FTO- Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH.
Konjugation: Unconjugated
Alternative Synonym: Alpha-ketoglutarate-dependent dioxygenase FTO, KIAA1752
Klonalität: Monoclonal
Klon-Bezeichnung: [5-2H10]
NCBI: 79068
UniProt: Q9C0B1
Puffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Formulierung: Liquid
Target-Kategorie: Human
Antibody Type: Primary Antibody
Application Verdünnung: IF Microscopy: 1:100, WB: 1:1000
Anwendungsbeschreibung: Anti-FTO (Mouse) Antibody has been tested in Western Blotting and ICC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 58 kDa in size corresponding to FTO proteins in Western blots of rat testes lysa
Western Blot of Mouse Anti-FTO (Fat mass and obesity related protein) antibody. Lane 1: rat testes. Lane 2: none. Load: 10 µg per lane. Primary antibody: FTO antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~ 58kDa/~58kDa for Fto protein. Other band(s): none.