FTO Antibody, Clone: [5-2H10], Mouse, Monoclonal

Product Overview

• Article Name: FTO Antibody, Clone: [5-2H10], Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-301-D41
• Supplier Catalog Number: 200-301-D41
• Alternative Catalog Number: ROC-200-301-D41
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Anti-FTO Monoclonal Antibody was produced in mouse by repeated immunizations with FTO- Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH.
• Conjugation: Unconjugated
• Alternative Names: Alpha-ketoglutarate-dependent dioxygenase FTO, KIAA1752

Product Properties

• Clonality: Monoclonal
• Clone Designation: [5-2H10]
• NCBI: 79068
• UniProt: Q9C0B1
• Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Form: Liquid
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: IF Microscopy: 1:100, WB: 1:1000
• Application Notes: Anti-FTO (Mouse) Antibody has been tested in Western Blotting and ICC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 58 kDa in size corresponding to FTO proteins in Western blots of rat testes lysa

Images

Western Blot of Mouse Anti-FTO (Fat mass and obesity related protein) antibody. Lane 1: rat testes. Lane 2: none. Load: 10 µg per lane. Primary antibody: FTO antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~ 58kDa/~58kDa for Fto protein. Other band(s): none.