IDO1 Antibody, IgG3, Clone: [10.1], Mouse, Monoclonal

Artikelnummer: ROC-200-301-E57S
Artikelname: IDO1 Antibody, IgG3, Clone: [10.1], Mouse, Monoclonal
Artikelnummer: ROC-200-301-E57S
Hersteller Artikelnummer: 200-301-E57S
Alternativnummer: ROC-200-301-E57S
Hersteller: Rockland Immunochemicals
Wirt: Mouse
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human, Mouse
Immunogen: Anti-IDO1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with fragment of recombinant human and mouse IDO1 protein followed by hybridoma development.
Konjugation: Unconjugated
Alternative Synonym: mouse anti-IDO1 antibody, Ido, Indo, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase, Ido1, Ido-1
Klonalität: Monoclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
Klon-Bezeichnung: [10.1]
Isotyp: IgG3
NCBI: 3620
UniProt: P14902
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Human
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:5000-1:50000, IHC: 1:100-1:500, IF Microscopy: 1:50-1:100, IP: 10-100 µL, WB: 1:500-1:1500
Anwendungsbeschreibung: Anti-IDO1 antibody has been tested in ELISA, IP, and Western Blot. This antibody is suitable for use in IHC and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Mouse Anti-IDO1 Antibody. Lane 1: untreated HeLa cells (p/n W09-000-364). Lane 2: IFN-r treated HeLa cells. Load: 35 µg per lane. Primary antibody: IDO 1 Antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO (p/n B501-0500) overnight at 4C. Predicted/Observed size: 41-42 kDa, 41-42 kDa for IDO-1. Other band(s): none.