IDO1 Antibody, IgG3, Clone: [10.1], Mouse, Monoclonal

Product Overview

• Article Name: IDO1 Antibody, IgG3, Clone: [10.1], Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-301-E57S
• Supplier Catalog Number: 200-301-E57S
• Alternative Catalog Number: ROC-200-301-E57S
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human, Mouse
• Immunogen: Anti-IDO1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with fragment of recombinant human and mouse IDO1 protein followed by hybridoma development.
• Conjugation: Unconjugated
• Alternative Names: mouse anti-IDO1 antibody, Ido, Indo, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase, Ido1, Ido-1

Product Properties

• Clonality: Monoclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Clone Designation: [10.1]
• Isotype: IgG3
• NCBI: 3620
• UniProt: P14902
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5000-1:50000, IHC: 1:100-1:500, IF Microscopy: 1:50-1:100, IP: 10-100 µL, WB: 1:500-1:1500
• Application Notes: Anti-IDO1 antibody has been tested in ELISA, IP, and Western Blot. This antibody is suitable for use in IHC and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.

Images

Western Blot of Mouse Anti-IDO1 Antibody. Lane 1: untreated HeLa cells (p/n W09-000-364). Lane 2: IFN-r treated HeLa cells. Load: 35 µg per lane. Primary antibody: IDO 1 Antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO (p/n B501-0500) overnight at 4C. Predicted/Observed size: 41-42 kDa, 41-42 kDa for IDO-1. Other band(s): none.