Alpha B Crystallin Antibody, IgG1, Clone: [1A7.D5], Mouse, Monoclonal

Artikelnummer: ROC-200-301-E85
Artikelname: Alpha B Crystallin Antibody, IgG1, Clone: [1A7.D5], Mouse, Monoclonal
Artikelnummer: ROC-200-301-E85
Hersteller Artikelnummer: 200-301-E85
Alternativnummer: ROC-200-301-E85
Hersteller: Rockland Immunochemicals
Wirt: Mouse
Kategorie: Antikörper
Applikation: IF, WB
Spezies Reaktivität: Bovine, Human
Immunogen: Alpha B Crystallin Antibody was produced in mice by repeated immunizations with Native Alpha B Crystallin protein.
Konjugation: Unconjugated
Alternative Synonym: AACRYA, CRYA2, CRYAB, CTPP2, HspB5, NY Ren 27 antigen antibody, alpha crystallin B antibody, Heat shock protein beta-5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component
Klonalität: Monoclonal
Konzentration: 1mg/mL by UV absorbance at 280 nm
Klon-Bezeichnung: [1A7.D5]
Isotyp: IgG1
NCBI: 1410
UniProt: P02511
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Human
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:200, WB: 1:2000
Anwendungsbeschreibung: Anti-Alpha B Crystallin Antibody has been tested in WB and IF and is recommended for use in ELISA. Expect a band approximately ~20kDa (Predicted mol. weight is ~21kDa) corresponding to alphaB-crystallin. Specific conditions for reactivity should be optimized
Western Blot of mouse Anti-Alpha B Crystallin Antibody. Lane 1: AlphaA Crystallin. Lane2: AlphaB Crystallin. Load: 20ug per lane. Primary antibody: Alpha B-Crystallin at 1:1000 for overnight at 4C . Secondary antibody: Goat anti-Mouse IgG HRP antibody at 1:40,000 for 45 min at RT. Block: 5% Blotto overnight at 4C. Predicted/Observed size: 19.9 kDa, 20 kDa for Alpha B.