Alpha B Crystallin Antibody, IgG1, Clone: [1A7.D5], Mouse, Monoclonal

Product Overview

• Article Name: Alpha B Crystallin Antibody, IgG1, Clone: [1A7.D5], Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-301-E85
• Supplier Catalog Number: 200-301-E85
• Alternative Catalog Number: ROC-200-301-E85
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: IF, WB
• Species Reactivity: Bovine, Human
• Immunogen: Alpha B Crystallin Antibody was produced in mice by repeated immunizations with Native Alpha B Crystallin protein.
• Conjugation: Unconjugated
• Alternative Names: AACRYA, CRYA2, CRYAB, CTPP2, HspB5, NY Ren 27 antigen antibody, alpha crystallin B antibody, Heat shock protein beta-5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component

Product Properties

• Clonality: Monoclonal
• Concentration: 1mg/mL by UV absorbance at 280 nm
• Clone Designation: [1A7.D5]
• Isotype: IgG1
• NCBI: 1410
• UniProt: P02511
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:200, WB: 1:2000
• Application Notes: Anti-Alpha B Crystallin Antibody has been tested in WB and IF and is recommended for use in ELISA. Expect a band approximately ~20kDa (Predicted mol. weight is ~21kDa) corresponding to alphaB-crystallin. Specific conditions for reactivity should be optimized

Images

Western Blot of mouse Anti-Alpha B Crystallin Antibody. Lane 1: AlphaA Crystallin. Lane2: AlphaB Crystallin. Load: 20ug per lane. Primary antibody: Alpha B-Crystallin at 1:1000 for overnight at 4C . Secondary antibody: Goat anti-Mouse IgG HRP antibody at 1:40,000 for 45 min at RT. Block: 5% Blotto overnight at 4C. Predicted/Observed size: 19.9 kDa, 20 kDa for Alpha B.