GABA-A Receptor Alpha 1 protein Antibody was produced in mice by repeated immunizations with fusion protein corresponding to a near c-terminal region of mouse GABA-A-R-alpha1.
IHC: 0.1-1.0 µg/mL, IF Microscopy: 1.0-10 µg/mL, WB: 1 µg/mL
Anwendungsbeschreibung:
Anti-GABA-A receptor alpha 1 Antibody is tested for use in WB, IHC, and IF microscopy. Expect a band approximately ~55kDa on specific lysates. Antibody is provided in PBS pH 7.4. Specific conditions for reactivity should be optimized by the end user.
Western Blot of mouse anti-GABA-A Receptor ALPHA 1 protein antibody. Lane 1: human cell line mix. Lane 2: none. Load: 35 µg per lane. Primary antibody: GABA-A Receptor ALPHA 1 protein antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 54.1 kDa, ~55 kDa for GABA-A Receptor ALPHA 1 protein. Other band(s): none.
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