GABA-A Receptor alpha 1 Antibody, IgG1, Clone: [N95/35], Mouse, Monoclonal

Catalog Number: ROC-200-301-F30
Article Name: GABA-A Receptor alpha 1 Antibody, IgG1, Clone: [N95/35], Mouse, Monoclonal
Biozol Catalog Number: ROC-200-301-F30
Supplier Catalog Number: 200-301-F30
Alternative Catalog Number: ROC-200-301-F30
Manufacturer: Rockland Immunochemicals
Host: Mouse
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: GABA-A Receptor Alpha 1 protein Antibody was produced in mice by repeated immunizations with fusion protein corresponding to a near c-terminal region of mouse GABA-A-R-alpha1.
Conjugation: Unconjugated
Alternative Names: Gamma-aminobutyric acid receptor subunit alpha-1, GABA(A) receptor subunit alpha-1, Gabra-1
Clonality: Monoclonal
Concentration: 1.0 mg/mL by UV absorbance at 280 nm
Clone Designation: [N95/35]
Isotype: IgG1
NCBI: 14394
UniProt: P62812
Buffer: See application note.
Form: Liquid (sterile filtered)
Target: Mouse
Antibody Type: Primary Antibody
Application Dilute: IHC: 0.1-1.0 µg/mL, IF Microscopy: 1.0-10 µg/mL, WB: 1 µg/mL
Application Notes: Anti-GABA-A receptor alpha 1 Antibody is tested for use in WB, IHC, and IF microscopy. Expect a band approximately ~55kDa on specific lysates. Antibody is provided in PBS pH 7.4. Specific conditions for reactivity should be optimized by the end user.
Western Blot of mouse anti-GABA-A Receptor ALPHA 1 protein antibody. Lane 1: human cell line mix. Lane 2: none. Load: 35 µg per lane. Primary antibody: GABA-A Receptor ALPHA 1 protein antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 54.1 kDa, ~55 kDa for GABA-A Receptor ALPHA 1 protein. Other band(s): none.