GABA-A receptor-delta Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to an internal region of rat GABA-A-R-Delta.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Rat
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:5000-1:25,000, IHC: 0.1-1.0ug/mL, IF Microscopy: 1.0-10ug/mL, WB: 1-10ug/mL
Anwendungsbeschreibung:
Anti-GABA-A receptor delta Antibody is tested for use in WB and IF microscopy. Expect a band approximately ~55kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Western Blot of mouse anti-GABA-A Receptor Delta antibody. Lane 1: Rat cell line mix. Load: 15 µg per lane. Primary antibody: GABA-A Receptor Delta antibody at 1:1000 for 2hr at RT. Secondary antibody: Sheep Anti-mouse HRP secondary antibody at 1:10,000 for 60 min at RT. Block: 1.5% BSA for 30 min at RT. Predicted/Observed size: 50.6 kDa, ~53 kDa for GABA-A Receptor Delta. Other band(s): none.
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