GABA-A Receptor delta Antibody, IgG2a, Clone: [N151/3], Mouse, Monoclonal

Product Overview

• Article Name: GABA-A Receptor delta Antibody, IgG2a, Clone: [N151/3], Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-301-F33
• Supplier Catalog Number: 200-301-F33
• Alternative Catalog Number: ROC-200-301-F33
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: IF, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: GABA-A receptor-delta Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to an internal region of rat GABA-A-R-Delta.
• Conjugation: Unconjugated
• Alternative Names: GABAA-RD, Gabrd, MGC105467, Gamma-aminobutyric acid receptor subunit delta, GABA(A) receptor subunit delta

Product Properties

• Clonality: Monoclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Clone Designation: [N151/3]
• Isotype: IgG2a
• NCBI: 29689
• UniProt: P18506
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Rat
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5000-1:25,000, IHC: 0.1-1.0ug/mL, IF Microscopy: 1.0-10ug/mL, WB: 1-10ug/mL
• Application Notes: Anti-GABA-A receptor delta Antibody is tested for use in WB and IF microscopy. Expect a band approximately ~55kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Images

Western Blot of mouse anti-GABA-A Receptor Delta antibody. Lane 1: Rat cell line mix. Load: 15 µg per lane. Primary antibody: GABA-A Receptor Delta antibody at 1:1000 for 2hr at RT. Secondary antibody: Sheep Anti-mouse HRP secondary antibody at 1:10,000 for 60 min at RT. Block: 1.5% BSA for 30 min at RT. Predicted/Observed size: 50.6 kDa, ~53 kDa for GABA-A Receptor Delta. Other band(s): none.