0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:200, WB: 1 µg/ml
Anwendungsbeschreibung:
Anti-Hsp90alphabeta Antibody is tested for WB, ELISA, IF, and IHC. Expect a band approximately 90kD proteins corresponding to the molecular mass of hsp90 alpha or beta on SDS Page immunoblots. Specific conditions for reactivity should be optimized by the end user.
Western Blot of mouse anti-Hsp90 total antibody. Lane 1: A431. Lane 2: A549. Lane 3: HCT116. Lane 4: HeLa. Lane 5: HEK293. Lane 6: HepG2. Lane 7: HL-60. Lane 8: HUVEC. Lane 9: Jurkat. Lane 10: MCF7. Lane 11: PC3. Lane 12: T98G. Lane 13: Rat Brain. Load: 10 µg per lane. Primary antibody: Hsp90 total antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 84.7 kDa/~90for Hsp90 total. Other band(s): none.
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