HSP90 alpha/beta Antibody, IgG2a, Clone: [Hyb-K41220A], Mouse, Monoclonal

Product Overview

• Article Name: HSP90 alpha/beta Antibody, IgG2a, Clone: [Hyb-K41220A], Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-301-F75
• Supplier Catalog Number: 200-301-F75
• Alternative Catalog Number: ROC-200-301-F75
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Rat, Yeast
• Immunogen: Hsp90 alpha beta Antibody was produced in mice by repeated immunizations raised against recombinant human Hsp90alpha.
• Conjugation: Unconjugated
• Alternative Names: Anti-Hsp90alphabeta, Hsp86, Hsp89A, Hsp90AA1, Hsp90Alpha, HspC1, HSPCA, HspCAL3, Heat shock protein HSP 90-alpha, Heat shock 86 kDa, HSP 86, HSP86, Renal carcinoma antigen NY-REN-38, HSP90AA1, HSP90A, HSPC1, HSPCA

Product Properties

• Clonality: Monoclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Clone Designation: [Hyb-K41220A]
• Isotype: IgG2a
• NCBI: 3320
• UniProt: P07900
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:200, WB: 1 µg/ml
• Application Notes: Anti-Hsp90alphabeta Antibody is tested for WB, ELISA, IF, and IHC. Expect a band approximately 90kD proteins corresponding to the molecular mass of hsp90 alpha or beta on SDS Page immunoblots. Specific conditions for reactivity should be optimized by the end user.

Images

Western Blot of mouse anti-Hsp90 total antibody. Lane 1: A431. Lane 2: A549. Lane 3: HCT116. Lane 4: HeLa. Lane 5: HEK293. Lane 6: HepG2. Lane 7: HL-60. Lane 8: HUVEC. Lane 9: Jurkat. Lane 10: MCF7. Lane 11: PC3. Lane 12: T98G. Lane 13: Rat Brain. Load: 10 µg per lane. Primary antibody: Hsp90 total antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 84.7 kDa/~90for Hsp90 total. Other band(s): none.