0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:200, IP: User Optimized, WB: 1:1000
Anwendungsbeschreibung:
Anti-p38 MAP kinase Antibody is tested for use in WB, IP, IHC, and ELISA. Expect a band approximately ~38kDa protein corresponding to the molecular mass of p38alpha MAPK on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the e
Western Blot of mouse anti-p38 alpha antibody. Lane 1: A431. Lane 2: A549. Lane 3: HCT116. Lane 4: HeLa. Lane :5 HEK293. Lane 6: HepG2. Lane 7: HL-60. Lane 8:HUVEC. Lane 9: Jurkat. Lane 10: MCF7. Lane 11: PC3. Lane 12: T98G. Lane 13: Rat Brain. Primary antibody: p38 alpha antibody at 1:1000 for overnight at 4C. Secondary antibody: Goat anti-mouse IgG HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blotto overnight 4C. Predicted/Observed size: 41.2kDa/38kD. Other band(s): none.
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