p38 alpha MAPKinase Antibody, IgG1, Clone: [9F12], Mouse, Monoclonal

Product Overview

• Article Name: p38 alpha MAPKinase Antibody, IgG1, Clone: [9F12], Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-301-G27
• Supplier Catalog Number: 200-301-G27
• Alternative Catalog Number: ROC-200-301-G27
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: IHC, IP, WB
• Species Reactivity: Human
• Immunogen: p38 Antibody was produced in mice by repeated immunizations raised against a full length recombinant protein expressed in E.coli cells.
• Conjugation: Unconjugated
• Alternative Names: CSAID Binding protein 1, CSBP1, CSBP2, EXIP, MAP kinase MXI2, MAPkinase p38alpha, p38 ALPHA, p38 MAP kinase, p38 mitogen activated protein kinase, RK, SAPK 2A, Stress activated protein kinase 2A, Mitogen-activated protein kinase 14, Cytokine suppressive anti-inflammatory drug-binding protein

Product Properties

• Clonality: Monoclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Clone Designation: [9F12]
• Isotype: IgG1
• NCBI: 1432
• UniProt: Q16539
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:200, IP: User Optimized, WB: 1:1000
• Application Notes: Anti-p38 MAP kinase Antibody is tested for use in WB, IP, IHC, and ELISA. Expect a band approximately ~38kDa protein corresponding to the molecular mass of p38alpha MAPK on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the e

Images

Western Blot of mouse anti-p38 alpha antibody. Lane 1: A431. Lane 2: A549. Lane 3: HCT116. Lane 4: HeLa. Lane :5 HEK293. Lane 6: HepG2. Lane 7: HL-60. Lane 8:HUVEC. Lane 9: Jurkat. Lane 10: MCF7. Lane 11: PC3. Lane 12: T98G. Lane 13: Rat Brain. Primary antibody: p38 alpha antibody at 1:1000 for overnight at 4C. Secondary antibody: Goat anti-mouse IgG HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blotto overnight 4C. Predicted/Observed size: 41.2kDa/38kD. Other band(s): none.