Ubiquitin Antibody, IgG2a, Clone: [6C11-B3], Mouse, Monoclonal

Produktübersicht

• Artikelname: Ubiquitin Antibody, IgG2a, Clone: [6C11-B3], Mouse, Monoclonal
• Artikelnummer: ROC-200-301-G62
• Hersteller Artikelnummer: 200-301-G62
• Alternativnummer: ROC-200-301-G62
• Hersteller: Rockland Immunochemicals
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: IF, WB
• Spezies Reaktivität: Bovine, Human, Mouse, Rat
• Immunogen: Ubiquitin Antibody was produced in mice by repeated immunizations raised against native bovine ubiquitin conjugated to KLH.
• Konjugation: Unconjugated
• Alternative Synonym: Polyubiquitin B, RPS27A, UBA52, UBB, UBC, ubiquitin B

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1mg/mL by UV absorbance at 280 nm
• Klon-Bezeichnung: [6C11-B3]
• Isotyp: IgG2a
• NCBI: 281370
• UniProt: P0CG53
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Bovine
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:200, WB: 1:1000
• Anwendungsbeschreibung: Anti-Ubiquitin Antibody is tested for use in WB and IF. Expect a band approximately ~10kDa corresponding to free ubiquitin. Specific conditions for reactivity should be optimized by the end user.

Bilder

Western Blot of mouse anti-Ubiquitin antibody. Lane 1: Human cell lines. Primary antibody: Ubiquitin antibody at 1:1000 for overnight at 4C. Secondary antibody: Goat anti-mouse IgG HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blotto overnight 4C. Predicted/Observed size: 25.7 kDa/10kD free ubiquitin. Other band(s): none.