Ubiquitin Antibody, IgG2a, Clone: [6C11-B3], Mouse, Monoclonal

Product Overview

• Article Name: Ubiquitin Antibody, IgG2a, Clone: [6C11-B3], Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-301-G62
• Supplier Catalog Number: 200-301-G62
• Alternative Catalog Number: ROC-200-301-G62
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: IF, WB
• Species Reactivity: Bovine, Human, Mouse, Rat
• Immunogen: Ubiquitin Antibody was produced in mice by repeated immunizations raised against native bovine ubiquitin conjugated to KLH.
• Conjugation: Unconjugated
• Alternative Names: Polyubiquitin B, RPS27A, UBA52, UBB, UBC, ubiquitin B

Product Properties

• Clonality: Monoclonal
• Concentration: 1mg/mL by UV absorbance at 280 nm
• Clone Designation: [6C11-B3]
• Isotype: IgG2a
• NCBI: 281370
• UniProt: P0CG53
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Bovine
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:200, WB: 1:1000
• Application Notes: Anti-Ubiquitin Antibody is tested for use in WB and IF. Expect a band approximately ~10kDa corresponding to free ubiquitin. Specific conditions for reactivity should be optimized by the end user.

Images

Western Blot of mouse anti-Ubiquitin antibody. Lane 1: Human cell lines. Primary antibody: Ubiquitin antibody at 1:1000 for overnight at 4C. Secondary antibody: Goat anti-mouse IgG HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blotto overnight 4C. Predicted/Observed size: 25.7 kDa/10kD free ubiquitin. Other band(s): none.