PARP Antibody, IgG2b, Clone: [194C1439], Mouse, Monoclonal

Produktübersicht

• Artikelname: PARP Antibody, IgG2b, Clone: [194C1439], Mouse, Monoclonal
• Artikelnummer: ROC-200-301-I03
• Hersteller Artikelnummer: 200-301-I03
• Alternativnummer: ROC-200-301-I03
• Hersteller: Rockland Immunochemicals
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC, IF, WB
• Spezies Reaktivität: Human
• Immunogen: PARP Antibody was produced in mice prepared by repeated immunizations with a peptide containing amino acids near the 214/215 cleavage site of human PARP protein.
• Konjugation: Unconjugated
• Alternative Synonym: ADPRT, PPOL, Poly [ADP-ribose] polymerase 1, PARP-1, 2.4.2.30, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 0.5 mg/mL by UV absorbance at 280 nm
• Klon-Bezeichnung: [194C1439]
• Isotyp: IgG2b
• NCBI: 142
• UniProt: P09874
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: Flow Cytometry: 1-3 µg/test, WB: 1-2 µg/mL
• Anwendungsbeschreibung: Anti-PARP antibody is tested for use in WB, Flow, Flow-IC, and ICC/IF. Expect a band approximately 89kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Bilder

Western Blot of Mouse Anti-PARP antibody. Lane 1-4hr: Cleaved PARP in staurosporine-treated Jurkat cells. Primary antibody: PARP antibody at 2 µg/mL for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 80 kDa for PARP. Other band(s): none.