PARP Antibody, IgG2b, Clone: [194C1439], Mouse, Monoclonal

Product Overview

• Article Name: PARP Antibody, IgG2b, Clone: [194C1439], Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-301-I03
• Supplier Catalog Number: 200-301-I03
• Alternative Catalog Number: ROC-200-301-I03
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: FC, IF, WB
• Species Reactivity: Human
• Immunogen: PARP Antibody was produced in mice prepared by repeated immunizations with a peptide containing amino acids near the 214/215 cleavage site of human PARP protein.
• Conjugation: Unconjugated
• Alternative Names: ADPRT, PPOL, Poly [ADP-ribose] polymerase 1, PARP-1, 2.4.2.30, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1

Product Properties

• Clonality: Monoclonal
• Concentration: 0.5 mg/mL by UV absorbance at 280 nm
• Clone Designation: [194C1439]
• Isotype: IgG2b
• NCBI: 142
• UniProt: P09874
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: Flow Cytometry: 1-3 µg/test, WB: 1-2 µg/mL
• Application Notes: Anti-PARP antibody is tested for use in WB, Flow, Flow-IC, and ICC/IF. Expect a band approximately 89kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Images

Western Blot of Mouse Anti-PARP antibody. Lane 1-4hr: Cleaved PARP in staurosporine-treated Jurkat cells. Primary antibody: PARP antibody at 2 µg/mL for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 80 kDa for PARP. Other band(s): none.