SARS-CoV-2 Spike Protein (RBD) Antibody, Clone: [15C11.A4 .H11.G7], Mouse, Monoclonal

Artikelnummer: ROC-200-301-MS8
Artikelname: SARS-CoV-2 Spike Protein (RBD) Antibody, Clone: [15C11.A4 .H11.G7], Mouse, Monoclonal
Artikelnummer: ROC-200-301-MS8
Hersteller Artikelnummer: 200-301-MS8
Alternativnummer: ROC-200-301-MS8
Hersteller: Rockland Immunochemicals
Wirt: Mouse
Kategorie: Antikörper
Applikation: ELISA
Spezies Reaktivität: Virus
Immunogen: This protein A purified antibody was produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the SARS Coronavirus Spike Protein within the Receptor Binding Domain (RBD).
Konjugation: Unconjugated
Alternative Synonym: 2019-nCoV, COVID-19, S glycoprotein, SARS CoV2 antibody, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2), Spike glycoprotein antibody, mouse anti-SARS CoV 2 Spike Protein Receptor Binding Domain Antibody, sars-cov-2
Klonalität: Monoclonal
Konzentration: 1.11 mg/mL by UV absorbance at 280 nm
Klon-Bezeichnung: [15C11.A4 .H11.G7]
NCBI: 908947
UniProt: P0DTC2
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Liquid (sterile filtered)
Target-Kategorie: SARS
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:200,000 - 1:325,000
Anwendungsbeschreibung: Anti-SARS-CoV-2 Spike protein purified antibody has been tested for use in ELISA and shows reactivity to recombinant SARS COV-2 Receptor Binding Domain. Specific conditions for reactivity should be optimized by the end user.
ELISA Results of Mouse Anti-SARS CoV-2 Spike Protein (RBD) Antibody. Each well was coated in duplicate with 0.5µg of BSA conjugated SARS-CoV-2 RBD (C-Term) [RED line], BSA conjugated SARS-CoV-2 RBD (N-Term) [GREEN line], and Recombinant SARS CoV-2 S1 [BLUE line]. The working dilution for BSA conjugated SARS-Cov-2 (C-Term) is 1:242,000 and BSA conjugated SARS-Cov-2 (N-Term) is 1:378,800. The starting dilution of antibody was 0.55µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. SARS-CoV-2 (C-Term) EC50: 4ng/mL and SARS-CoV-2 (N-Term) EC50: 3ng/mL.