SARS-CoV-2 Spike Protein (RBD) Antibody, Clone: [15C11.A4 .H11.G7], Mouse, Monoclonal

Product Overview

• Article Name: SARS-CoV-2 Spike Protein (RBD) Antibody, Clone: [15C11.A4 .H11.G7], Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-301-MS8
• Supplier Catalog Number: 200-301-MS8
• Alternative Catalog Number: ROC-200-301-MS8
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: ELISA
• Species Reactivity: Virus
• Immunogen: This protein A purified antibody was produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the SARS Coronavirus Spike Protein within the Receptor Binding Domain (RBD).
• Conjugation: Unconjugated
• Alternative Names: 2019-nCoV, COVID-19, S glycoprotein, SARS CoV2 antibody, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2), Spike glycoprotein antibody, mouse anti-SARS CoV 2 Spike Protein Receptor Binding Domain Antibody, sars-cov-2

Product Properties

• Clonality: Monoclonal
• Concentration: 1.11 mg/mL by UV absorbance at 280 nm
• Clone Designation: [15C11.A4 .H11.G7]
• NCBI: 908947
• UniProt: P0DTC2
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: SARS
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:200,000 - 1:325,000
• Application Notes: Anti-SARS-CoV-2 Spike protein purified antibody has been tested for use in ELISA and shows reactivity to recombinant SARS COV-2 Receptor Binding Domain. Specific conditions for reactivity should be optimized by the end user.

Images

ELISA Results of Mouse Anti-SARS CoV-2 Spike Protein (RBD) Antibody. Each well was coated in duplicate with 0.5µg of BSA conjugated SARS-CoV-2 RBD (C-Term) [RED line], BSA conjugated SARS-CoV-2 RBD (N-Term) [GREEN line], and Recombinant SARS CoV-2 S1 [BLUE line]. The working dilution for BSA conjugated SARS-Cov-2 (C-Term) is 1:242,000 and BSA conjugated SARS-Cov-2 (N-Term) is 1:378,800. The starting dilution of antibody was 0.55µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. SARS-CoV-2 (C-Term) EC50: 4ng/mL and SARS-CoV-2 (N-Term) EC50: 3ng/mL.