Paxillin Antibody, IgG1, Clone: [PXC-10], Mouse, Monoclonal

Artikelnummer: ROC-200-301-P44
Artikelname: Paxillin Antibody, IgG1, Clone: [PXC-10], Mouse, Monoclonal
Artikelnummer: ROC-200-301-P44
Hersteller Artikelnummer: 200-301-P44
Alternativnummer: ROC-200-301-P44
Hersteller: Rockland Immunochemicals
Wirt: Mouse
Kategorie: Antikörper
Applikation: WB
Spezies Reaktivität: Gallus, Human, Mouse, Rat
Immunogen: Paxillin antibody was produced in mice by repeated immunizations with recombinant protein corresponding to the C-terminal part of chicken paxillin (amino acids 305-559).
Konjugation: Unconjugated
Alternative Synonym: Paxillin antibody, PXN
Klonalität: Monoclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
Klon-Bezeichnung: [PXC-10]
Isotyp: IgG1
NCBI: 5829
UniProt: P49023
Puffer: 1.2% sodium acetate
Formulierung: Lyophilized
Target-Kategorie: Chicken
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:20,000, WB: 1-2ug/ml
Anwendungsbeschreibung: Anti-Paxillin is useful for Immunocytochemistry and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~64.5 kDa corresponding to the appropriate cell lysate or extract.
Western Blot of Mouse Anti-Paxillin antibody. Lane 1:293T Cell Lysate. Lane 2: HELA Cell Lysate. Lane3: MCF-7 Cell Lysate. Lane4:M231 Cell Lysate. Lane5: Jurkat Cell Lysate. Load: 35 µg per lane. Primary antibody: Paxillin antibody at 1-2ug/ml for overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:10,000 for 1.5 hr at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~64.5 kDa for Paxillin. Other band(s): none.