Paxillin Antibody, IgG1, Clone: [PXC-10], Mouse, Monoclonal

Product Overview

• Article Name: Paxillin Antibody, IgG1, Clone: [PXC-10], Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-301-P44
• Supplier Catalog Number: 200-301-P44
• Alternative Catalog Number: ROC-200-301-P44
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: WB
• Species Reactivity: Gallus, Human, Mouse, Rat
• Immunogen: Paxillin antibody was produced in mice by repeated immunizations with recombinant protein corresponding to the C-terminal part of chicken paxillin (amino acids 305-559).
• Conjugation: Unconjugated
• Alternative Names: Paxillin antibody, PXN

Product Properties

• Clonality: Monoclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Clone Designation: [PXC-10]
• Isotype: IgG1
• NCBI: 5829
• UniProt: P49023
• Buffer: 1.2% sodium acetate
• Form: Lyophilized
• Target: Chicken
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000, WB: 1-2ug/ml
• Application Notes: Anti-Paxillin is useful for Immunocytochemistry and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~64.5 kDa corresponding to the appropriate cell lysate or extract.

Images

Western Blot of Mouse Anti-Paxillin antibody. Lane 1:293T Cell Lysate. Lane 2: HELA Cell Lysate. Lane3: MCF-7 Cell Lysate. Lane4:M231 Cell Lysate. Lane5: Jurkat Cell Lysate. Load: 35 µg per lane. Primary antibody: Paxillin antibody at 1-2ug/ml for overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:10,000 for 1.5 hr at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~64.5 kDa for Paxillin. Other band(s): none.