Anti-Carboxypeptidase B antibody has been tested by ELISA and western blot and is suitable for Immunohistochemistry applications. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Rabbit Anti-Carboxypeptidase B antibody. Lane 1: Carboxypeptidase B. Lane 2: None. Load: 50 ng per lane. Primary antibody: Carboxypeptidase B primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 35 kDa, 35 kDa for Carboxypeptidase B. Other band(s): Carboxypeptidase B splice variants and isoforms.
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