Carboxypeptidase B Antibody, Rabbit, Polyclonal

Catalog Number: ROC-200-401-049-0100
Article Name: Carboxypeptidase B Antibody, Rabbit, Polyclonal
Biozol Catalog Number: ROC-200-401-049-0100
Supplier Catalog Number: 200-401-049-0100
Alternative Catalog Number: ROC-200-401-049-0100
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Porcine
Immunogen: Carboxypeptidase B [Porcine Pancreas]
Conjugation: Unconjugated
Alternative Names: rabbit anti-Carboxypeptidase B antibody, CarboxypeptidaseB1 antibody, CP B1 antibody, CPB 1 antibody, CPB antibody, CPB1 antibody, DKFZp779K1333 antibody, Pancreas specific protein antibody, Pancreatic carboxypeptidase B1 antibody
Clonality: Polyclonal
Concentration: 1.0 mg/ml by UV absorbance at 280 nm
NCBI: 397341
UniProt: P09955
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Lyophilized
Target: Swine
Antibody Type: Primary Antibody
Application Dilute: ELISA: 1:2,000 - 1:10,000, IHC: 1:250 - 1:1,000, WB: 1:500- 1:2,000
Application Notes: Anti-Carboxypeptidase B antibody has been tested by ELISA and western blot and is suitable for Immunohistochemistry applications. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Rabbit Anti-Carboxypeptidase B antibody. Lane 1: Carboxypeptidase B. Lane 2: None. Load: 50 ng per lane. Primary antibody: Carboxypeptidase B primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 35 kDa, 35 kDa for Carboxypeptidase B. Other band(s): Carboxypeptidase B splice variants and isoforms.