0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Bovine
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:5,000 - 1:20,000, WB: 1:1,000 - 1:5,000
Anwendungsbeschreibung:
Anti-Trypsin Antibody has been tested by western blotting and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Western Blot of Rabbit anti-Trypsin antibody. Lane 1: Trypsin (Bovine). Load: 50 ng per lane. Primary antibody: Trypsin antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 25 kDa for Trypsin. Other band(s): Low molecular weight bands could correspond to autocatalytic fragments.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten