Trypsin Antibody, Rabbit, Polyclonal

Catalog Number: ROC-200-401-139S
Article Name: Trypsin Antibody, Rabbit, Polyclonal
Biozol Catalog Number: ROC-200-401-139S
Supplier Catalog Number: 200-401-139S
Alternative Catalog Number: ROC-200-401-139S
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Bovine
Immunogen: Anti-Trypsin Antibody was produced by repeated immunizations with bovine pancreatic trypsin protein purified.
Conjugation: Unconjugated
Alternative Names: rabbit anti-trypsin antibody, Anionic trypsinogen antibody, Beta trypsin antibody, Brain trypsinogen antibody, Cationic Trypsinogen antibody, Digestive Zymogen antibody, Mesotrypsin antibody, Mesotrypsinogen antibody
Clonality: Polyclonal
Concentration: 1.0 mg/mL by UV absorbance at 280 nm
NCBI: 780933
UniProt: P00760
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Liquid (sterile filtered)
Target: Bovine
Antibody Type: Primary Antibody
Application Dilute: ELISA: 1:5,000 - 1:20,000, WB: 1:1,000 - 1:5,000
Application Notes: Anti-Trypsin Antibody has been tested by western blotting and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Western Blot of Rabbit anti-Trypsin antibody. Lane 1: Trypsin (Bovine). Load: 50 ng per lane. Primary antibody: Trypsin antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 25 kDa for Trypsin. Other band(s): Low molecular weight bands could correspond to autocatalytic fragments.