0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
WB: 2-5 µg/mL
Anwendungsbeschreibung:
Anti-SMAD7 antibody is tested for use in WB, ELISA, and IHC. Expect a band approximately 46kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Rabbit Anti-SMAD7 MADH7 antibody. Lane A: Lysate from HepG2. Primary antibody: SMAD7 MADH7 at 5 µg/mL for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 80 kDa for SMAD7 MADH7. Other band(s): none.
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