SMAD7 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: SMAD7 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-200-401-I16
• Supplier Catalog Number: 200-401-I16
• Alternative Catalog Number: ROC-200-401-I16
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: SMAD7 Antibody was produced from whole rabbit serum prepared by repeated immunizations with SMAD7 peptide.
• Conjugation: Unconjugated
• Alternative Names: MADH7, MADH8

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/mL by UV absorbance at 280 nm
• NCBI: 4092
• UniProt: O15105
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB: 2-5 µg/mL
• Application Notes: Anti-SMAD7 antibody is tested for use in WB, ELISA, and IHC. Expect a band approximately 46kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Images

Western Blot of Rabbit Anti-SMAD7 MADH7 antibody. Lane A: Lysate from HepG2. Primary antibody: SMAD7 MADH7 at 5 µg/mL for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 80 kDa for SMAD7 MADH7. Other band(s): none.