CLIC1 Antibody, Rabbit, Polyclonal

Artikelnummer: ROC-200-401-MP3S
Artikelname: CLIC1 Antibody, Rabbit, Polyclonal
Artikelnummer: ROC-200-401-MP3S
Hersteller Artikelnummer: 200-401-MP3S
Alternativnummer: ROC-200-401-MP3S
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IP, WB
Spezies Reaktivität: Human
Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with human CLIC1 recombinant protein.
Konjugation: Unconjugated
Alternative Synonym: Rabbit Anti-CLIC1 Antibody, Chloride Intracellular Channel 1, Regulatory Nuclear Chloride Ion Channel Protein, Chloride Intracellular Channel Protein 1, Nuclear Chloride Ion Channel 27, Chloride Channel ABP, NCC27, HRNCC, G6, Nuclear Chloride Ion Channel Protein, RNCC Protein, P64CLCP
Klonalität: Polyclonal
Konzentration: 1.06 mg/mL by UV absorbance at 280 nm
NCBI: 1192
UniProt: O00299
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Human
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: User Optimized, IP: User Optimized, WB: 1 µg/mL
Anwendungsbeschreibung: Anti-CLIC1 has been tested in ELISA, Western Blot, and Immunoprecipitation. Expect a band at ~27kda in western blot using appropriate tissues and lysates. Positive controls used in western blot include CLIC1 overexpressing HEK293, WM-115, and HL-60 whole
Western Blot of Rabbit Anti-CLIC1 Antibody. Lane 1: Opal Prestained Molecular Weight Markers (p/n MB-210-0500). Lane 2: CLIC1 Overexpressed HEK293 Lysate (10µg) [+]. Lane 3: WM115 Lysate (p/n W09-001-GY8) (35µg) [+]. Lane 4: HL-60 Lysate (p/n W09-001-GK8) (35µg) [+]. Lane 5: Human Kidney Lysate (35µg) [+]. Lane 6: HeLa WCL Lysate (p/n W09-000-364) (35µg) [-]. Lane 7: Human Cerebellum Lysate (35µg) [-]. Primary Antibody: Anti-CLIC1 at 1µg/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:70,000 for 1hr at RT. Block: BlockOut buffer (p/n MB-073). Expect MW: ~27kDa. Exposure: 22 sec.