CLIC1 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: CLIC1 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-200-401-MP3S
• Supplier Catalog Number: 200-401-MP3S
• Alternative Catalog Number: ROC-200-401-MP3S
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IP, WB
• Species Reactivity: Human
• Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with human CLIC1 recombinant protein.
• Conjugation: Unconjugated
• Alternative Names: Rabbit Anti-CLIC1 Antibody, Chloride Intracellular Channel 1, Regulatory Nuclear Chloride Ion Channel Protein, Chloride Intracellular Channel Protein 1, Nuclear Chloride Ion Channel 27, Chloride Channel ABP, NCC27, HRNCC, G6, Nuclear Chloride Ion Channel Protein, RNCC Protein, P64CLCP

Product Properties

• Clonality: Polyclonal
• Concentration: 1.06 mg/mL by UV absorbance at 280 nm
• NCBI: 1192
• UniProt: O00299
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: User Optimized, IP: User Optimized, WB: 1 µg/mL
• Application Notes: Anti-CLIC1 has been tested in ELISA, Western Blot, and Immunoprecipitation. Expect a band at ~27kda in western blot using appropriate tissues and lysates. Positive controls used in western blot include CLIC1 overexpressing HEK293, WM-115, and HL-60 whole

Images

Western Blot of Rabbit Anti-CLIC1 Antibody. Lane 1: Opal Prestained Molecular Weight Markers (p/n MB-210-0500). Lane 2: CLIC1 Overexpressed HEK293 Lysate (10µg) [+]. Lane 3: WM115 Lysate (p/n W09-001-GY8) (35µg) [+]. Lane 4: HL-60 Lysate (p/n W09-001-GK8) (35µg) [+]. Lane 5: Human Kidney Lysate (35µg) [+]. Lane 6: HeLa WCL Lysate (p/n W09-000-364) (35µg) [-]. Lane 7: Human Cerebellum Lysate (35µg) [-]. Primary Antibody: Anti-CLIC1 at 1µg/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:70,000 for 1hr at RT. Block: BlockOut buffer (p/n MB-073). Expect MW: ~27kDa. Exposure: 22 sec.