0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Lyophilized
Antibody Type:
Primary Antibody
Application Verdünnung:
Flow Cytometry: User Optimized, IF Microscopy: 1:500-1:2,500
Anwendungsbeschreibung:
Anti-Streptavidin Antibody Fluorescein Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging,
Rockland Rabbit anti Streptavidin (200-4195 lot 23495) and Biotin conjugated Rabbit anti-trypsin inhibitor antibody (200-4679 lot 6594) were used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of primary antibody (ON 4 C). Detection shown was using Dylight 649 conjugated Donkey anti rabbit (611-743-127 lot 20831 1:10K 1.5 hr RT in MB-070) and imaged on the BioRad VersaDoc System
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
