Streptavidin Antibody Fluorescein Conjugated, FITC, Rabbit, Polyclonal Preis auf Anfrage

Product Overview

• Article Name: Streptavidin Antibody Fluorescein Conjugated, FITC, Rabbit, Polyclonal Preis auf Anfrage
• Biozol Catalog Number: ROC-200-4295
• Supplier Catalog Number: 200-4295
• Alternative Catalog Number: ROC-200-4295
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Species Reactivity: Bacteria
• Immunogen: Streptavidin (Streptomyces avidinii)
• Conjugation: FITC
• Alternative Names: rabbit anti-Streptavidin Antibody FITC Conjugation, Fluorescein conjugated rabbit anti-Streptavidin antibody, Anti-Streptavidin FITC Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 10.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: Flow Cytometry: User Optimized, IF Microscopy: 1:500-1:2,500
• Application Notes: Anti-Streptavidin Antibody Fluorescein Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging,

Images

Rockland Rabbit anti Streptavidin (200-4195 lot 23495) and Biotin conjugated Rabbit anti-trypsin inhibitor antibody (200-4679 lot 6594) were used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of primary antibody (ON 4 C). Detection shown was using Dylight 649 conjugated Donkey anti rabbit (611-743-127 lot 20831 1:10K 1.5 hr RT in MB-070) and imaged on the BioRad VersaDoc System