0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Chicken
Antibody Type:
Primary Antibody
Application Verdünnung:
Flow Cytometry: User Optimized, IP: User Optimized, WB: 0.5µg/ml
Anwendungsbeschreibung:
Anti-Grp94 Antibody is tested for WB, IF, IP, and Flow cytometry. Expect a band approximately 98kDa protein corresponding to the molecular mass of Grp94 on SDS PAGE immunoblots. This monoclonal antibody reacts with purified Grp94. Specific conditions for
Western Blot of rat anti-Grp94 antibody. Lane 1: molecular weight. Lane 2: HeLa Lysates. Load: 35 µg per lane. Primary antibody: Grp94 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rat secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 92.5 kDa, ~110 kDa for Grp94. Other band(s): none.
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