GRP94 Antibody, IgG2a, Clone: [9G10], Rat, Monoclonal

Product Overview

• Article Name: GRP94 Antibody, IgG2a, Clone: [9G10], Rat, Monoclonal
• Biozol Catalog Number: ROC-200-501-F39
• Supplier Catalog Number: 200-501-F39
• Alternative Catalog Number: ROC-200-501-F39
• Manufacturer: Rockland Immunochemicals
• Host: Rat
• Category: Antikörper
• Application: FC, IF, IP, WB
• Species Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus
• Immunogen: Grp94 Antibody was produced in rat by repeated immunizations with purified Grp94 isolated from chicken oviducts.
• Conjugation: Unconjugated
• Alternative Names: HSP90B1, GP96, TRA1, ECGP, Endoplasmin, 94 kDa glucose-regulated protein, GRP-94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1, gp96 homolog

Product Properties

• Clonality: Monoclonal
• Concentration: 1mg/mL by UV absorbance at 280 nm
• Clone Designation: [9G10]
• Isotype: IgG2a
• NCBI: 374163
• UniProt: P08110
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Chicken
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: Flow Cytometry: User Optimized, IP: User Optimized, WB: 0.5µg/ml
• Application Notes: Anti-Grp94 Antibody is tested for WB, IF, IP, and Flow cytometry. Expect a band approximately 98kDa protein corresponding to the molecular mass of Grp94 on SDS PAGE immunoblots. This monoclonal antibody reacts with purified Grp94. Specific conditions for

Images

Western Blot of rat anti-Grp94 antibody. Lane 1: molecular weight. Lane 2: HeLa Lysates. Load: 35 µg per lane. Primary antibody: Grp94 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rat secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 92.5 kDa, ~110 kDa for Grp94. Other band(s): none.