0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:200, WB: 1:1000
Anwendungsbeschreibung:
Anti-Hsp70 Antibody is tested for use in WB and IF. Specific conditions for reactivity should be optimized by the end user.
Western Blot of chicken anti-Hsp70 antibody. Lane 1: HeLa Cell Lysate. Lane 2: none. Load: 35 µg per lane. Primary antibody: Hsp70 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) chicken secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 70.1 kDa/~70 kDa for Hsp70. Other band(s): none.
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