Hsp70 Antibody, Gallus, Polyclonal

Artikelnummer: ROC-200-901-F63
Artikelname: Hsp70 Antibody, Gallus, Polyclonal
Artikelnummer: ROC-200-901-F63
Hersteller Artikelnummer: 200-901-F63
Alternativnummer: ROC-200-901-F63
Hersteller: Rockland Immunochemicals
Wirt: Gallus
Kategorie: Antikörper
Applikation: IF, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Hsp70 Antibody was produced in chicken prepared by repeated immunizations raised against full length protein Hsp70.
Konjugation: Unconjugated
Alternative Synonym: Heat shock 70 kDa protein, HSP70
Klonalität: Polyclonal
Konzentration: 1mg/mL by UV absorbance at 280 nm
NCBI: 3303
UniProt: P0DMV8
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Human
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:200, WB: 1:1000
Anwendungsbeschreibung: Anti-Hsp70 Antibody is tested for use in WB and IF. Specific conditions for reactivity should be optimized by the end user.
Western Blot of chicken anti-Hsp70 antibody. Lane 1: HeLa Cell Lysate. Lane 2: none. Load: 35 µg per lane. Primary antibody: Hsp70 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) chicken secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 70.1 kDa/~70 kDa for Hsp70. Other band(s): none.