Hsp70 Antibody, Gallus, Polyclonal

Catalog Number: ROC-200-901-F63
Article Name: Hsp70 Antibody, Gallus, Polyclonal
Biozol Catalog Number: ROC-200-901-F63
Supplier Catalog Number: 200-901-F63
Alternative Catalog Number: ROC-200-901-F63
Manufacturer: Rockland Immunochemicals
Host: Gallus
Category: Antikörper
Application: IF, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Hsp70 Antibody was produced in chicken prepared by repeated immunizations raised against full length protein Hsp70.
Conjugation: Unconjugated
Alternative Names: Heat shock 70 kDa protein, HSP70
Clonality: Polyclonal
Concentration: 1mg/mL by UV absorbance at 280 nm
NCBI: 3303
UniProt: P0DMV8
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Liquid (sterile filtered)
Target: Human
Antibody Type: Primary Antibody
Application Dilute: ELISA: 1:200, WB: 1:1000
Application Notes: Anti-Hsp70 Antibody is tested for use in WB and IF. Specific conditions for reactivity should be optimized by the end user.
Western Blot of chicken anti-Hsp70 antibody. Lane 1: HeLa Cell Lysate. Lane 2: none. Load: 35 µg per lane. Primary antibody: Hsp70 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) chicken secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 70.1 kDa/~70 kDa for Hsp70. Other band(s): none.