Beta-Galactosidase Antibody, Gallus, Polyclonal

Produktübersicht

• Artikelname: Beta-Galactosidase Antibody, Gallus, Polyclonal
• Artikelnummer: ROC-200-901-Z56
• Hersteller Artikelnummer: 200-901-Z56
• Alternativnummer: ROC-200-901-Z56
• Hersteller: Rockland Immunochemicals
• Wirt: Gallus
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Bacteria
• Immunogen: Anti-beta-gal antibody was prepared from chicken egg fractions produced by repeated immunizations with a 17 amino acid synthetic peptide from near the N-terminus of E. coli beta-gal.
• Konjugation: Unconjugated
• Alternative Synonym: beta-Galactosidase Antibody, Beta-galactosidase, Lactase, Beta-gal

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/mL by UV absorbance at 280 nm
• NCBI: 1036228
• UniProt: Q8FKG6
• Puffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Bacillus
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000, WB: 1 µg/mL
• Anwendungsbeschreibung: Anti-beta-Galactosidase Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 116 kDa in Western Blots of specific cell lysates and tissues.

Bilder

Western Blot of b-gal antibody. Lane 1: 5 ng of b-gal with b-gal antibody at 1 µg/mL. Lane 2: 25 ng of b-gal with b-gal antibody at 1 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase chicken secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 116 kDa, 120 kDa for b-gal. Other band(s): b-gal splice variants and isoforms.