Rabbit IgG anti-Dog IgG (F(ab)2)-HRPO, MinX none

Artikelnummer: ROC-204-4304
Artikelname: Rabbit IgG anti-Dog IgG (F(ab)2)-HRPO, MinX none
Artikelnummer: ROC-204-4304
Hersteller Artikelnummer: 204-4304
Alternativnummer: ROC-204-4304
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA,IHC,WB
Spezies Reaktivität: Canine
Immunogen: Dog IgG F(ab)2 fragment
Konjugation: HRPO
Alternative Synonym: rabbit anti-Dog IgG F(ab)2 Antibody Peroxidase Conjugated, rabbit anti-Dog IgG Fab2 HRP Conjugated Antibody
Format: IgG
Spezifität: IgG (F(ab')2)
Minimale Kreuzreaktivität (MinX): no cross-adsorbtion
Klonalität: Polyclonal
Konzentration: 10.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Dog
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
Anwendungsbeschreibung: Anti-Dog IgG F(ab)2 Peroxidase Conjugate has been tested by ELISA. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis
ELISA results of purified Rabbit Anti-Dog IgG F(ab)2 Peroxidase Antibody tested against purified Dog IgG Fab2. Each well was coated in duplicate with 1.0 µg of Dog IgG F(ab)2 (red line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB-1000 substrate.