Rabbit IgG anti-Dog IgG (F(ab)2)-HRPO, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Dog IgG (F(ab)2)-HRPO, MinX none
• Biozol Catalog Number: ROC-204-4304
• Supplier Catalog Number: 204-4304
• Alternative Catalog Number: ROC-204-4304
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Canine
• Immunogen: Dog IgG F(ab)2 fragment
• Conjugation: HRPO
• Alternative Names: rabbit anti-Dog IgG F(ab)2 Antibody Peroxidase Conjugated, rabbit anti-Dog IgG Fab2 HRP Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (F(ab')2)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 10.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Dog
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Application Notes: Anti-Dog IgG F(ab)2 Peroxidase Conjugate has been tested by ELISA. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis

Images

ELISA results of purified Rabbit Anti-Dog IgG F(ab)2 Peroxidase Antibody tested against purified Dog IgG Fab2. Each well was coated in duplicate with 1.0 µg of Dog IgG F(ab)2 (red line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB-1000 substrate.