Anti-Dog IgM mu chain Peroxidase Conjugated has been tested dot blot and ELISA. This product is assayed against 1.0 ug of Dog IgM in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrat
Dot Blot results of Rabbit Anti-Dog IgM Antibody Peroxidase Conjugated. Dots are Dog IgM at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 30 min at RT. Primary Antibody: none. Secondary Antibody: Rabbit Anti-Dog IgM-HRP at 1µg/mL for 1hr at RT. Imaged with BioRad ChemiDoc, Chemi filter.
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